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Fig. 3 | Parasites & Vectors

Fig. 3

From: Identification of a novel methyltransferase-type 12 protein from Haemonchus contortus and its effects on functions of goat PBMCs

Fig. 3

Expression, purification and immunoreactivity of rHcMTF-12. a Lane M: standard protein molecular weight marker; Lane 0: recombinant expression vector before induction; Lane 1–7: protein expression induced with 1 mM IPTG. b Lane 1: purified rHcMTF-12 protein resolved on SDS-PAGE. c Lane M: molecular weight protein ladder; Lane 1: purified rHcMTF-12 (transferred to a membrane probed with serum from goat infected with H. contortus); Lane 2: membrane probed with normal goat serum as control. d Lane 3: total ES proteins of H. contortus probed with antibodies from SD rats immunized with rHcMTF-12; Lane 4: membrane probed with normal rat sera as control

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Parasites & Vectors

ISSN: 1756-3305

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