Fig. 2

Culture conditions of N. brasiliensis iL3 and RNA-Seq experimental design. Rats were experimentally infected with iL3; after patency (1 week), stool was collected for three consecutive days and larvae cultured for two weeks at 26 °C. Subsequently, iL3 were isolated and cultured for 24 h with either Δ7-dafachronic acid (DA) or vehicle (ethanol) at 20 °C, 26 °C or 37 °C for both treatments. Each condition was performed in triplicate, with sample A derived from the stool culture on the first day of collection, sample B on the second day, and sample C on the third day. Total RNA was then isolated from iL3 and used to construct RNA-Seq libraries