Table 1 Results of molecular analyses for piroplasm DNA in Stomoxys calcitrans according to collection site, month and sex
From: DNA of Theileria orientalis, T. equi and T. capreoli in stable flies (Stomoxys calcitrans)
Locality | Habitat characteristics | Month | PCR-positive among all tested | Result of sequencing | GenBank ID | ||
|---|---|---|---|---|---|---|---|
Males | Females | Species (n) | Identity (%) | ||||
1 | Stable (void of cattle for > 2 h) | August | 0/2 | 1/14 | T. orientalis (1×) | 100 | MN611176 |
September | 20/55 | 2/4 | T. orientalis (22×) | 100 | MN611176 | ||
October | 14/30 | 15/45 | T. orientalis (29×) | 100 | MN611176 | ||
Pen on pasture (with cattle) | August | 1/2 | 2/14 | T. capreolia (1×) | 100 | MN611178 | |
T. orientalis (2×) | 100 | MN611176 | |||||
September | 11/22 | 2/10 | T. orientalis (13×) | 100 | MN611176 | ||
October | 14/31 | 5/9 | T. orientalis (19×) | 100 | MN611176 | ||
2 | Farm with horses, game | October | 0/2 | 2/21 | T. equi (2×) | 100 | MN611177 |
3 | Farm with cattle | October | 0/3 | 0/5 | – | – | – |
4 | Lake shore (beach) | August | 0/1 | 0/3 | – | – | – |
5 | Garden (in capital city) | November | 0/1 | – | – | – | – |
6 | Garden (in a village) | September | 0/1 | – | – | – | – |
7 | Livestock reserve with horses and ruminants | June | 1/25 | 0/59 | T. equi (1×) | 100 | MN611177 |
8 | Garden with a horse | July | 1/4 | 1/5 | T. equi (2×) | 100 | MN611177 |
9 | Garden (in a village) | September | 0/2 | – | – | – | – |
10 | Garden (in a village) | September | 0/17 | 0/8 | – | – | – |