Plasmodium vivax HAP2/GCS1 gene exhibits limited genetic diversity among parasite isolates from the Greater Mekong Subregion

Table 1 Mutations and corresponding amino acid substitutions in the pvhap2 gene

Nucleotide position	Codon	Wild-type	Mutant	Amino acid	Frequency (%)
Nucleotide position	Codon	Wild-type	Mutant	Amino acid	China-Myanmar border (n = 135)	Myanmar (n = 51)	Thailand (n = 41)
77	26	GAT	GTT	D – V	0.74	0	0
78	26	GAT	GAA	D – E	0	0	2.44
183	61	GGG	GGC	G – G	0	3.92	0
479	129	AAG	AAC	K – N	37.04	7.84	0
487	133	TAC	TCC	Y – S	99.26	100	95.12
626	179	AGG	AGA	R – R	0	1.96	0
759	224	CAA	AAA	Q – K	1.48	0	0
797	236	GAC	GAT	D – D	0	1.96	0
1037	316	CAA	CAG	Q – Q	0	0	2.44
1038	317	GAG	AAG	E – K	0	0	2.44
1374	429	TTG	CTG	L – L	5.19	3.92	2.44
1624	513	TGC	TGT	C – C	0	1.96	0
1714	542	GGG	GAG	G – E	0	0	2.44
1808	573	TTA	TTG	L – L	0	1.96	0
1813	575	AAC	AGC	N – S	0	1.96	0
1998	637	GAT	AAT	D – N	82.96	36.54	68.29
2009	640	ACG	ACA	T – T	0.74	1.96	4.88
2139	684	GCC	GTC	A – V	0	1.96	0
2140	684	GCC	TCC	A – S	0	1.96	0
2226	713	GCC	ACC	A – T	0.74	1.96	0
2320	744	GGG	GTG	G – V	0	1.96	0
2357	759	ACG	ACA	V – V	0.74		2.44
2372	761	TCC	TCT	S – S	0	1.96	0
2384	765	AAA	AAG	K – K	0		2.44
2628	847	GGC	AGC	G – S	0	3.92	0
2636	849	CGA	CGC	R – R	100	100	100
2640	851	CCC	ACC	P – T	0	3.92	0
2649	854	GCA	ACA	A – T	1.48	1.96	0

Notes: The nucleotides and amino acid of synonymous mutation sites are indicated in bold

ISSN: 1756-3305