Table 1 PCR primers used for detection of selected vector-borne pathogens: Ehrlichia spp., Anaplasma spp., Hepatozoon canis, Babesia spp., Theileria spp. and Leishmania spp. in dogs from Kathmandu, Nepal
From: Stray dogs in Nepal have high prevalence of vector-borne pathogens: a molecular survey
Species | Target gene | Forward primer (5’-3’) | Reverse primer (5’- 3’) | References |
|---|---|---|---|---|
Ehrlichia spp.; Anaplasma spp. | 16S rRNA | GCAAGCYTAACACATGCAAGTCG | GGATTATACAGTATTACCCAYCATTTCTARTG | [6] |
Hepatozoon canis | 18S rRNA | CTTACCGTGGCAGTGACGGT | ATTGTTATTTCTTGTTACTACCTCTCTCAAAC | |
Piroplasmida (Babesia/Theileria spp.) | GACGATCAGATACCGTCGTAGTCC | CAGAACCCAAAGACTTTGATTTCTCTC | ||
Leishmania spp. | Kinetoplast minicircle DNA | AACTTTTCTGGTCCTCCGGGTAG | ACCCCCAGTTTCCCGCC | [7] |