A phylogenetic analysis of the biting midges belonging to Culicoides Latreille (Diptera: Ceratopogonidae) subgenus Avaritia using molecular data

Table 1 Primers used to amplify the five molecular regions of Culicoides specimens

Gene	Primer name	Sequence 5′–3′	Fragment length (bp)	Reference
cox1	C1J1718	GGAGGATTTGGAAATTGATTAGT	523	Dallas et al. [38]
	C1N2191	CAGGTAAAATTAAAATATAAACTTCTGG		Dallas et al. [38]
cox2	COIIF20	ATGGCAACTTGAGGAMATAT	601	This study
	COIIR612	CGCAGATTTCTGAACATTG		This study
cytb	CytbF373	ATAGGAACTGCTTTTATAGG	526	This study
	CytbR944	CAATAGATATGACTAAAGCGATTACT		This study
ITS1-5.8S-ITS2	PanCulF	GTAGGTGAACCTGCGGAAGG	~785	Cêtre-Sossah et al. [67]
	28SR	ATTTGGGGGTAGTCACACAT		Gomulski et al. [40]

Note: The 3 regions ITS1-58S-ITS2 were amplified together
Abbreviations: cox1, cytochrome c oxidase subunit 1; cox2, cytochrome c oxidase 2; cytb for cytochrome b; ITS1-58S-ITS2, internal transcribed spacer 1-5.8S-internal transcribed spacer 2

ISSN: 1756-3305