Fig. 5

Immunoblot results for antibodies against N. caninum on serum samples from a llama experimentally inoculated with 4.8 × 10E6 cell culture-derived N. caninum tachyzoites (Llama 1) and from a seronegative control llama inoculated with 5 × 10E4 Vero cells (Llama 2). Abbreviations: M, marker; kDa, kilodaltons; 2–52 (indicated above), days post-inoculation (dpi). Bands corresponding to immunodominant N. caninum antigens (17, 29, 30, 33 and 37 kDa) are indicated with an asterisk. In Llama 1, reaction against the 29 kDa antigen is observed at 12 dpi. From 16 to 52 dpi also further immunodominant antigens are recognized. Samples recognizing two or more immunodominant antigens were considered positive. Llama 2 did not recognize any of the N. caninum immunodominant antigens during the observation period