Fig. 3

Phylogenetic analysis of B. caballi isolated from clinically infected horses (diamonds) and subclinically infected horses (open squares) (sample names as detailed in Additional file 1: Table S1). a Analysis of 1212 nucleotide positions of B. caballi 18S rRNA gene sequences from six clinical samples along with additional published sequences (GenBank ID/parasite/host/location). The phylogenetic tree was constructed based on the Tamura-Nei model with gamma distribution (+ G) and invariable sites (+ I). The percentage of trees in which the associated samples clustered together is shown next to the branches when it was above 70%. The analysis was constructed in MEGA7. b Analysis of 251 nucleotide positions of B. caballi rap-1 gene sequences from six clinical and 13 subclinical samples, along with additional sequences from GenBank. The phylogenetic tree was based on the Kimura 2-parameter model with consideration on invariable sites (+ I). Both phylogenetic trees were constructed using maximum likelihood method and 1000 bootstrap replicates. The percentage of trees in which the associated samples clustered together is shown next to the branches when it was above 70%. The analysis was constructed in MEGA7