Fig. 2
Identification of differentially expressed proteins between the MTZ-S and MTZ-R proteomes. To determine the concentration and incubation period of MTZ that could differentiate the growth patterns between MTZ-S (ATCC 30236) and MTZ-R (ATCC 50143) parasites, the cell density of trophozoites treated with different concentrations of MTZ was monitored for 24 h. The initial concentration of both MTZ-S and MTZ-R strains was 2 × 105 cells/ml. MTZ-S trophozoites (a) and MTZ-R trophozoites (b) were treated with MTZ (5 μM, 10 μM or 20 μM) compared with the SDW-treated control (Ctrl). The cell density was monitored every 4 h by trypan blue exclusion assay. The growth curves were presented as mean ± SD of three independent experiments. c Whole cell lysates extracted from MTZ-S and MTZ-R parasites in the presence or absence of MTZ were separated with 10% SDS-PAGE followed by Coomassie blue staining. d A total of 3123 and 3125 proteins were identified in the paired MTZ-R and MTZ-S proteomes treated with or without MTZ, respectively. Among these identified proteins, 228 and 76 proteins were upregulated and downregulated, respectively, in the MTZ-R proteome treated with MTZ. Additionally, 120 and 124 proteins were upregulated and downregulated respectively, in the MTZ-R proteome without MTZ treatment