Fig. 5
From: Glucose transporter GLUT1 influences Plasmodium berghei infection in Anopheles stephensi
Transcriptome analysis of dsRNA treated An. stephensi infected with P. berghei. a Venn diagram showing overlapping genes between dsGFP- and dsAsteglut1-treated groups. b Heatmap of the differentially expressed genes in An. stephensi midguts infected with P. berghei. Upregulated genes are shown in red; downregulated genes are shown in green (P < 0.05; fold change > 2). c Pie charts showing the distribution of upregulated genes (left) and downregulated genes (right). d Melanization (left panel) and melanization rate (right panel) of P. berghei in dsRNA-treated mosquitoes. Live Plasmodium oocysts are shown in green and melanized ookinetes are indicated by arrows. Melanization rate was calculated by the ratio of the number of melanized ookinetes to the number of live Plasmodium oocysts observed per midgut. Results from one of two independent experiments are shown. Error bars indicate mean ± SEM (n = 35). Significance was determined by Student’s t-test (for details, see Additional file 4: Text S1). Abbreviation: NS, non-significant. Scale-bars: d, 50 μm