Fig. 2
From: Sirolimus enhances the protection achieved by a DNA vaccine against Leishmania infantum
Leishmania infantum DNA load. qPCR analyses showing the number of parasites detected per μg of DNA extracted from the spleen (a), liver (b), lymph node (c) and skin (d) comparing the three Leishmania-infected studied groups: C (PBS-inoculated control group, n =10), V (DNA vaccinated group, n = 10), and S + V (sirolimus-treated and DNA vaccinated group, n = 10). Parasite number was calculated by interpolation to a standard curve constructed with a L. infantum promastigote culture (e). Abbreviations: Cq, quantification cycle. ANOVA; *P < 0.05, **P < 0.01, ***P < 0.001