Table 2 Sequences of primers used for expressed cytokines detection
From: Sirolimus enhances the protection achieved by a DNA vaccine against Leishmania infantum
Gene | Primer sequence (5ʹ-3ʹ) | |
|---|---|---|
RPL18 | F | GTCCCTGTCCCGGATGATC |
R | GACAGTCCCCACAACCACG | |
IFN-γ | F | CTATGTCTGGCTGCTACTGCCA |
R | TTCACGACATCTAAGCTACTTGAGTTAA | |
TNF-α | F | CCAGACACTCACACTCAGATCATCTT |
R | CACTTGGTGGTTTGCTACAACGT | |
TGF-β | F | CTACCACGCCAACTTCTGTCTG |
R | TGTAGAGGGCAAGGACCTTACTG | |
FoxP3 | F | CAAGTGGCCTGGTTGTGAGA |
R | TGATCTGCTTGGCAGTGCT’ | |
IL-21 | F | CCAGCTCCACAAGATGTAAAGGA |
R | GCTGACTTGAGTTTGGCCTTCT | |
IL-10 | F | CATCGATTTCTCCCCTGTGAA |
R | GACGCCTTTCTCTTGGAGCTT | |
NOS2 | F | CCAGAACAGAGGGCTCAAAGG |
R | CCTGCATCTCTTCCCGATAGAG | |