Fig. 1

Bayesian inference trees of blackflies, based on 590 bp of the mitochondrial cox1 gene, and Leucocytozoon lineages carried by these blackflies, based on 504 bp of the mitochondrial cytb gene. Blackfly haplotypes are grouped into species (BLAST hits with > 98.5% sequence similarity). Lines connect Leucocytozoon lineages and the respective blackfly species in which they were detected. Line thickness is scaled to the number of occurrences. An additional sequence from GenBank was added to species represented by single individuals for better representation. Node support is given for some branches and is based on 1000 bootstrap replicates. † The clade also includes one sequence of S. aureum; * S. vernum, S. naturale and S. cryophilum are indistinguishable based on the sequenced cox1 fragment and are denoted as S. vernum*