Fig. 3

Excretory/secretory products of Angiostrongylus cantonensis L5 stimulate astrocyte activation and GRP78 expression. Cells were treated with 0, 31.3, 62.5, 125 or 250 μg/ml excretory/secretory products (ESPs) of A. cantonensis L5 for 12 h. The mRNA (a) and protein (b) expression levels of GFAP and GRP78 were detected by real-time qPCR and western blotting. The data are expressed as the mean ± SD from three independent experiments (n = 3). *P < 0.05 and **P < 0.01 compared with cells treated with 0 μg/ml ESPs of A. cantonensis L5 for 12 h