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Table 5 Distribution of kdr genotype-combinations with respect to locus S989, V1016 and F1534 in dead and alive mosquitoes (after 1 h exposure and 24 h recovery period) and their genetic association with insecticide resistance phenotype

From: A new knockdown resistance (kdr) mutation, F1534L, in the voltage-gated sodium channel of Aedes aegypti, co-occurring with F1534C, S989P and V1016G

Insecticide

Dead/alive

Statistical tests

Genotypes with respect to S989, V1016 and F1534

SSVVFF

SSVVFC

SSVVFL

SSVVCC

SSVVLL

SSVVLC

SPVGFF

SPVGFC

SPVGFL

PPGGFF

DDT 4%

Alive

 

3

36

2

46

0

21

16

48

7

8

Dead

 

2

8

0

0

0

0

1

3

1

1

 

Fisher’s exact

–

ns

nd

P < 0.01

nd

nd

nd

ns

nd

nd

 

OR (95% CI)

–

3.00 (0.43–21.01)

nd

Infinity

nd

nd

nd

10.67 (1.26–90.29)

nd

nd

PER 0.75%

Alive

 

2

15

6

79

10

63

1

24

13

11

Dead

 

4

27

3

16

3

15

8

4

2

3

 

Fisher’s exact

–

ns

nd

P < 0.05

nd

P < 0.05

nd

P < 0.05

nd

nd

 

OR (95% CI)

–

1.11 (0.18–6.80)

nd

9.87 (1.66–55.58)

nd

8.40 (1.4–50.22)

nd

12.00 (1.62–88.71)

nd

nd

DEL 0.05%

Alive

 

10

38

19

52

4

48

11

21

5

5

Dead

 

8

23

7

23

0

10

8

8

1

1

 

Fisher’s exact test

–

ns

ns

ns

nd

P < 0.05

ns

ns

nd

nd

 

OR (95% CI)

–

1.32 (0.46.3.83)

2.17 (0.60–7.74)

1.81 (0.63–5.17)

nd

3.84 (1.21–12.16)

1.10 (0.30–4.04)

2.10 (0.61–7.23)

nd

nd

  1. Abbreviations: OR, odds ratio; CI, confidence interval; DDT, dichloro diphenyl trichloroethane; PER, permethrin; DEL, deltamethrin; ns, not significant; nd, not determined