Bioassay and molecular monitoring of insecticide resistance status in Aedes albopictus populations from Greece, to support evidence-based vector control

Table 4 Genotype and allele frequencies (%) of VGSC domain II locus V1016 and domain III loci I1532 and F1534

Region	Year	V1016G		I1532T						F1534C
		N₁	% allele freq	N₂	Genotype			% allele freq		Genotype			% allele freq
		N₁	(V)	N₂	II	IT	TT	(I)	(T)	FF	FC	CC	(F)	(C)
Rodopi	2018	13	100	12	11	1	0	95.8	4.2	8	4	0	83.3	16.7
Thessaloniki
Diavata, Lagkadikia^b	2017	18^a	100	8	8	0	0	100	0	7	1	0	93.8	6.2
Thermi-Litsa	2018	30	100	30	28	2	0	96.7	3.3	26	4	0	93.3	6.7
Chios^b	2017	22^a	100	11	11	0	0	100	0	6	4	1	72.7	27.3
Attica	2018	59	100	52	52	0	0	100	0	12	9	31	31.7	68.3
Kefalonia	2019	45	100	41	41	0	0	100	0	37	1	3	91.5	8.5
Patras	2019	33	100	33	20	11	2	77.3	22.7	28	5	0	92.4	7.6
Argolida	2018	21	100	21	19	2	0	95.2	4.8	10	3	8	54.8	45.2
Kalamata	2018	7	100	6	6	0	0	100	0	2	3	1	58.3	41.7
Rethymno	2018	20	100	30	29	1	0	98.3	1.7	10	11	9	51.7	48.3
Chania	2018	31	100	31	27	4	0	93.5	6.5	15	14	2	71.0	29.0
Heraklion	2019	44	100	44	44	0	0	100	0	15	19	10	55.7	44.3

^aIncludes 10 eggs analysed in a pool. Results are presented cumulatively for the different sampling localities in each region, except for Thessaloniki ^bSamples from Diavata and Lagkadikia refugee camps (Thessaloniki) and Chios were analysed in Fotakis et al. [33]
Abbreviations: N₁ and N₂, total number of genotyped specimens for VGSC domain II and domain III respectively, per sampling region; V, 1016V susceptible allele. Genotypes for VGSC locus 1016 are not given as all samples were wild-type 1016V/1016V; I, 1532I susceptible allele; T, 1532T mutant allele; II, 1532I/1532I homozygous; IT, 1532I/1532T heterozygote; TT, 1532T/1532T homozygous mutant; F, 1534F susceptible allele; C, 1534C mutant allele; FF, 1534F/1534F homozygous; FC, 1534F/1534C heterozygote; CC, 1534C/1534C homozygous mutant

ISSN: 1756-3305