Table 2 Overview of molecular identifications. Table represents the 382 morphologically identified Anopheles specimens that were sequenced using the cox1 locus. Final species identifications are based on both cox1 BLASTn and BOLD database comparisons
Consensus sequence groups | n | Collection method HDT/HLC | BLASTn sequence homology (%ID) | BOLD sequence homology (%ID) | Tentative species ID | Final species ID |
|---|---|---|---|---|---|---|
Group 1 | 316 | 18/298 | An. barbirostris (99) | An. barbirostris (98–96) | An. barbirostris | An. barbirostris |
Group 2 | 32 | 4/28 | An. epiroticus (97) | An. sundaicus (s.l.) (98–96) | An. epiroticus/An. sundaicus (s.l.) | Sundaicus Complex |
Group 3 | 13 | 2/11 | An. epiroticus (97) | An. epiroticus (97–96) | An. epiroticus | An. epiroticus |
Group 4 | 7 | 0/7 | An. nitidus (96) | An. letifer (98–95) | An. nitidus/An. letifer | Unknown |
Group 5 | 5 | 0/5 | An. nigerrimus (96) | An. letifer (98–95) | An. nigerrimus/An. letifer | Unknown |
Group 6 | 3 | 0/3 | An. peditaeniatus (99) | An. peditaeniatus (100–99) | An. peditaeniatus | An. peditaeniatus |
Group 7 | 3 | 0/3 | An. vagus (95) | An. vagus (99–90) | An. vagus | An. vagus |
Group 8 | 2 | 1/1 | Cx. bitaeniorhynchus (100) | Cx. bitaeniorhynchus (100–94) | Cx. bitaeniorhynchus | Cx. bitaeniorhynchus |
Group 9 | 1 | 0/1 | Ae. vexans (99) | Aedimorphus vexans (99–97) | Ae. vexans | Ae. vexans |