Table 2 Proteins identified by 2D-DIGE/mass spectrometry as differentially expressed between intestinal epithelial cells of rIL-25-treated mice versus rIL-25-treated animals and exposed to metacerariae of Echinostoma caproni

Metabolic enzymes

 63

Pyruvate kinase PKM

Mus musculus

− 1.9

58/132

7.17/8.20

Glycolysis

Cyt; Nuc

51.34

52.54

30

 69

Pyruvate kinase PKM

Mus musculus

− 1.7

58/132

7.17/8.48

Glycolysis

Cyt; Nuc

46.03

42.94

26

 101

Glutamate dehydrogenase 1

Mus musculus

− 1.6

61/126

8.05/8.05

Glutamine anaplerosis

Mit

70.19

62.54

26

 123

6-Phodphogluconate dehydrogenase

Mus musculus

− 1.7

53/122

6.81/7.62

Pentose phosphate pathway

Cyt

14

18.84

65

 144

Enolase 1B

Mus musculus

− 1.8

47/118

6.37/8.46

Glycolysis

Cyt; PM; Mit

89.91

79.95

30

 154

Fumarate hydratase

Mus musculus

− 1.6

54/118

9.12/9.12

Krebs cycle

Mit

39.41

35.5

25

 156

Enolase 1B

Mus musculus

− 1.8

47/118

6.37/6.37

Glycolysis

Cyt; PM; Mit

46.26

51.15

35

 166

Creatine kinase B-type

Mus musculus

− 2.0

43/116

5.34/5.34

Cretine kinase activity

Cyt

12.4

29.92

7

 184

Phosphoglycerate kinase 1

Mus musculus

− 2.3

45/114

8.02/9.5

Glycolysis

Cyt

46.01

63.55

39

 214

Glyceraldehyde-3-phosphate dehydrogenase

Mus musculus

− 1.9

36/108

8.44/9.43

Glycolysis

Cyt

15.03

42.94

8

 226

Aspartate aminotransferase

Mus musculus

− 2.2

47/108

9.13/9.92

Aminoacid metabolism

Mit

48.42

50

39

 250

Transaldolase

Mus musculus

− 1.8

42/102

6.57/7.04

Pentose phosphate pathway

Cyt

20.35

35.34

16

 257

Malate dehydrogenase

Mus musculus

− 1.8

37/96

6.16/6.16

Krebs cycle

Cyt

28.05

41.62

19

 262

Glyceraldehyde-3-phosphate dehydrogenase

Mus musculus

+ 1.8

36/100

8.44/8.44

Glycolysis

Cyt

13.22

39.64

11

 279

Ornithine carbamoyltransferase

Mus musculus

− 2.0

39/100

8.81/8.30

Ornithine metabolism

Mit

35.79

45.87

55

 290

Palmitoyl-protein thioesterase

Mus musculus

− 2.6

35/94

8.26/8.85

Palmitoyl metabolism

Cyt

10,04

21.5

5

 296

Malate dehydrogenase

Mus musculus

− 1.8

37/94

6.16/6.28

Krebs cycle; malate shuttle

Cyt

61.69

74.55

70

 302

Malate dehydrogenase

Mus musculus

+ 2.2

37/90

6.16/5.58

Krebs cycle; malate shuttle

Cyt

17.01

36.53

10

 305

Malate dehydrogenase

Mus musculus

+ 1.7

37/90

6.16/5.98

Krebs cycle; malate shuttle

Cyt

42.04

53.59

45

 387

Triosephosphate isomerase

Mus musculus

− 1.8

32/60

5.56/8.66

Glycolysis; gluconeogenesis

Cyt

3.9

16.03

3

Structural proteins

 243

Junction plakoglobin

Mus musculus

− 2.3

82/104

5.75/6.43

Cell adhesión

Cyt

39.85

46.98

20

 260

Junction plakoglobin

Mus musculus

− 1.5

82/102

5.75/8.18

Cell adhesion

Cyt

29.38

29.4

18

 325

Junction plakoglobin

Mus musculus

− 1.6

82/86

5.75/6.34

Cell adhesion

Cyt

12.5

17.85

8

Antioxidant-detoxifying enzymes

 358

Dihydropteridine reductase

Mus musculus

− 2.5

22/74

7.67/8.40

Oxireductase activity

Mit

11.8

31.92

8

 418

Glutathione S-transferase P 1

Mus musculus

− 1.6

24/60

7.69/8.33

Glutathione conjugation and detoxification

Cyt; Mit; Nuc

18.06

57.14

35

 421

Glutathione S-transferase P 1

Mus musculus

− 1.7

24/59

7.69/9.21

Glutathione conjugation and detoxification

Cyt; Mit; Nuc

31.38

78.1

75

 431

Peroxiredoxin-1

Mus musculus

− 2.0

22/57

8.26/8.26

Redox regulation

Cyt

10.18

33.67

7

Calcium-binding proteins

 271

Annexin A2

Mus musculus

+ 1.6

39/101

7.55/8.84

Membrane transport; fibrin homeostasis

PM

39.75

71.98

18

 324

Annexin A4

Mus musculus

+ 2.5

36/86

5.43/5.02

Membrane transport

PM ext

23.72

41.69

15