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Fig. 4 | Parasites & Vectors

Fig. 4

From: Eimeria tenella Eimeria-specific protein that interacts with apical membrane antigen 1 (EtAMA1) is involved in host cell invasion

Fig. 4

Interaction between EtAMA1 and EtEsp in DF-1 cells assessed with BiFC. a DF-1 cells were transfected with VC155-EtAMA1 and VN155-EtEsp and the cellular lysates were analyzed with immunoblotting using antisera against EtAMA1 and EtEsp. Lane 1: anti-r EtAMA1 antibody; Lane 2, anti-r EtEsp antibody. b BiFC was performed. b1 DF-1 cells were co-transfected with VC155-EtAMA1 and VN155-EtEsp. b2 DF-1 cells were co-transfected with positive controls bFos and bJun. b3 DF-1 cells were co-transfected with pBiFC-VC155-EtAMA1 and pBiFC-VN155 empty vector. b4 DF-1 cells were co-transfected with pBiFC-VN155-EtEsp and pBiFC-VC155 empty vector

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Parasites & Vectors

ISSN: 1756-3305

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