Fig. 4From: The role of the Acanthamoeba castellanii Sir2-like protein in the growth and encystation of AcanthamoebaEffects of AcSir2 overexpression on Acanthamoeba trophozoites. a Wild-type (A. castellanii, black) and trophozoites overexpressing either EGFP (left, green) or AcSir2 (right, green) were analyzed on a flow cytometer for changes in cell size using the mean FSC-H. b The DNA content of the trophozoites overexpressing either EGFP (left) or AcSir2 (right) was determined after PI staining using flow cytometry. The data represent one of three experiments with similar results. c Ultrastructural changes in AcSir2-overexpressing cells. Trophozoites transfected with the vector control (pGAPDH-EGFP) (left) and pGAPDH-AcSir2-EGFP (right) were examined using transmission electron microscopy (TEM). Scale-bars: c, 1 μmBack to article page