Table 1 Summary of the characteristics of serological tests relative to two references of experimental chickens, (A) T. gondii inoculated vs non-inoculated chickens, (B) MC-qPCR (magnetic capture quantitative PCR) positive vs non-inoculated chickens, stratified for the tests. For the analysis, all experimentally inoculated chickens were excluded, if their infection had not been confirmed by MC-qPCR

(A) Inoculated vs non-inoculated

TgSAG1_bio-BBMA

98.5 [90.7–99.9] (65/66)

100 [82.2–100] (23/23)

0.99

TgSAG1-ELISA^c_SH

91.1 [80.6–96.3] (60/66)

100 [82.2–100] (23/23)

0.91

TgSAG1-ELISA^c_SL

92.4 [82.5–97.2] (61/66)

100 [82.2–100] (23/23)

0.92

IFAT^c

97.0 [88.5–99.5] (64/66)

100 [82.2–100] (23/23)

0.97

MAT^c

92.4 [82.5–97.2] (61/66)

100 [82.2–100] (23/23)

0.87

(B) MC-qPCR positive vs non-inoculated

TgSAG1_bio-BBMA

100 [90.4–100] (45/45)

100 [82.2–100] (23/23)

1.00

TgSAG1-ELISA^c_SH

100 [90.4–100] (45/45)

100 [82.2–100] (23/23)

1.00

TgSAG1-ELISA^c_SL

100 [90.4–100] (45/45)

100 [82.2–100] (23/23)

1.00

IFAT^c

100 [90.4–100] (45/45)

100 [82.2–100] (23/23)

1.00

MAT^c

100 [90.4–100] (45/45)

100 [82.2–100] (23/23)

1.00