Fig. 3
From: TgROP18 targets IL20RB for host-defense-related-STAT3 activation during Toxoplasma gondii infection
Identification of IL-20RB interacting with TgROP18 with FRET and Co-IP. a Identification of the interaction using FRET. The COS-7 cells co-transfected with pECFPN1-ROP18 and pEYFPC1-IL20RB (EXP) were used to detect the FRET efficiency between CFPN1-ROP18 and YFPC1-IL20RB. The COS-7 cells co-transfected with pECFPN1 and pEYFPC1 were used for negative control (NC) and the cells transfected with pECFPN1-EYFP as the positive control (PC). The FRET efficiency is shown in the right column where a color matched FRET efficiency scale is indicated for each image. The red end of the scale indicates a higher FRET efficiency. b The calculated FRET efficiency (left) and intermolecular distance (right) between ROP18 and IL20RB is shown as a column. c The interaction of ROP18 with IL20RB was further confirmed through immunoprecipitation assay (Co-IP) in vitro. Group 1: cells transfected with pEYFP-IL20RB-HA; Group 2: cells transfected with pCFP-ROP18I-3×FLAG; Group 3: cells co-transfected with pCFP-ROP18I-3×FLAG and pEYFP-IL20RB-HA; Group 4: negative control group