From: Detection of human intestinal protozoan parasites in vegetables and fruits: a review
Location | Detection method | Vegetable or fruit item | No. of samples tested | No. of positive samples (%) | Giardia duodenalis assemblages identified (n) | References |
---|---|---|---|---|---|---|
Bangladesh | Iodine and normal saline wet mount | Vegetables | 200 | 2 (1.0) | Â | [52] |
Brazil | PCR | Lettuce and chicory | 11 | 2 (18.2) | Assemblage BIV (2) | [39] |
Brazil | Immunofluorescence, PCR | Arugula | 4 | 2 (50.0) | Assemblage AII (2) | [28] |
Chives | 12 | 1 (8.3) | Assemblage AII (1) | |||
Crisp lettuce | 32 | 4 (12.5) | Assemblage AII (4) | |||
Greens collard | 24 | 1 (4.2) | Assemblage AI (1) | |||
Parsley | 12 | 2 (16.7) | Assemblage AII (2) | |||
Watercress | 12 | 4 (33.3) | Assemblage AII (4) | |||
Wild chicory | 12 | 2 (16.7) | Assemblage AII (2) | |||
Brazil | Semi-nested PCR | Regular lettuce | 60 | 8 (13.3) | Assemblage AI (4); Assemblage B (1); Assemblage E (1); N/D (2) | [44] |
Crisp lettuce | 100 | 5 (5.0) | Assemblage AI (2); N/D (3) | |||
Chicory | 60 | 5 (8.3) | Assemblage AI (3); N/D (2) | |||
Rocket | 20 | 1 (5.0) | N/D (1) | |||
Kale | 20 | 0 | Â | |||
Brazil | PCR | Vegetables | 21 | 10 (47.6) | Assemblage E (2); N/D (8) | [45] |
Brazil | Sediment being stained in Lugolʼs solution | Lettuce | 100 | 0 |  | [15] |
Coriander | 100 | 1 (1.0) | Â | |||
Costa Rica | Direct smear, followed by light microscopy | Cilantro leaves | 80 | 4 (5.0) | Â | [79] |
Cilantro roots | 80 | 2 (2.5) | Â | |||
Egypt | Lugol’s iodine stain | Lettuce | 101 | 16 (15.8) |  | [18] |
Watercress | 116 | 13 (11.2) | Â | |||
Parsley | 102 | 12 (11.8) | Â | |||
Green onion | 103 | 4 (3.9) | Â | |||
Leek | 108 | 2 (1.9) | Â | |||
Ethiopia | Lugol’s iodine stain | Fruits and vegetables | 360 | 27 (7.5) |  | [19] |
Ethiopia | Sediment smear under light microscope | Fruits and vegetables | 360 | 36 (10.0) | Â | [32] |
Ethiopia | Sediment smear under light microscope | Tomatoes | 45 | 1 (2.2) | Â | [43] |
Lettuce | 45 | 4 (8.8) | Â | |||
Carrot | 45 | 7 (15.6) | Â | |||
Cabbage | 45 | 8 (17.8) | Â | |||
Green pepper | 45 | 6 (13.3) | Â | |||
Avocado | 45 | 0 | Â | |||
Ethiopia | Sediment smear and Lugol’s iodine stain | Tomato | 100 | 0 |  | [14] |
Cabbage | 96 | 16 (16.7) | Â | |||
Green pepper | 66 | 4 (6.1) | Â | |||
Carrot | 62 | 4 (6.5) | Â | |||
Salad | 23 | 0 | Â | |||
Ghana | Lugol’s iodine stain | Cabbage | 90 | 5 (5.6) |  | [12] |
Green pepper | 55 | 3 (5.5) | Â | |||
Carrot | 47 | 4 (8.5) | Â | |||
Onion | 70 | 3 (4.3) | Â | |||
Tomato | 31 | 2 (6.5) | Â | |||
Lettuce | 102 | 5 (4.9) | Â | |||
India | DAPI-stain followed by fluorescence microscopy, and PCR | Cabbage | 47 | 1 (2.1) | Â | [13] |
Carrot | 25 | 1 (4.0) | Â | |||
Chili | 42 | 4 (9.5) | Â | |||
Coriander | 28 | 3 (10.7) | Â | |||
Cucumber | 52 | 1 (1.9) | Assemblage D (1) | |||
Tomatoes | 56 | 2 (3.6) | Assemblage A (2) | |||
Turnip | 3 | 1 (33.3) | Â | |||
Iran | Lugol’s iodine stain | Vegetables | 141 | 11 (7.8) |  | [84] |
Iran | Sediment smear under light microscopy | Leek | 30 | 3 (10.0) | Â | [42] |
Spring onion | 22 | 0 | Â | |||
Basil | 15 | 1 (6.7) | Â | |||
Parsley | 21 | 0 | Â | |||
Lettuce | 23 | 0 | Â | |||
Cress | 17 | 0 | Â | |||
Spearmint | 18 | 0 | Â | |||
Tarragon | 19 | 0 | Â | |||
Coriander | 24 | 0 | Â | |||
Radish | 29 | 0 | Â | |||
Italy | Lugolʼs iodine satin and PCR | Ready-to-eat packaged salad | 648 | 4 (0.6) | Assemblage A (4) | [23] |
Jordan | Lugol’s iodine stain | Lettuce | 30 | 7 (23.3) |  | [20] |
Tomato | 33 | 2 (6.1) | Â | |||
Parsley | 42 | 0 | Â | |||
Cucumber | 28 | 0 | Â | |||
Norway | Concentrated by IMS, and screening by light microscopy | Alfalfa sprouts | 16 | 0 | Â | [35] |
Dill | 7 | 2 (28.6) | Â | |||
Lettuce | 125 | 2 (1.6) | Â | |||
Mung bean sprouts | 149 | 3 (2.0) | Â | |||
Mushrooms | 55 | 0 | Â | |||
Parsley | 7 | 0 | Â | |||
Precut salad mix | 38 | 0 | Â | |||
Radish sprouts | 6 | 1 (16.7) | Â | |||
Raspberries | 10 | 0 | Â | |||
Strawberries | 62 | 2 (3.2) | Â | |||
Norway | Concentrated by IMS, and screening by light microscopy | Alfalfa | 16 | 0 | Â | [82] |
Mung bean | 149 | 3 (2.0) | Â | |||
Radish | 6 | 1 (16.7) | Â | |||
Saudi Arabia | Lugol’s iodine stain | Green onion | 50 | 0 |  | [17] |
Watercress | 50 | 0 | Â | |||
Lettuce | 50 | 0 | Â | |||
Cucumber | 50 | 0 | Â | |||
Cabbage | 50 | 0 | Â | |||
Pea | 50 | 0 | Â | |||
Tomato | 50 | 0 | Â | |||
Carrot | 50 | 4 (8.0) | Â | |||
Spain | Concentrated by IMS and stain cysts for immunofluorescence assay | Chinese cabbage | 6 | 2 (33.3) | Â | [11] |
Lollo rosso lettuce | 4 | 3 (75.0) | Â | |||
Romaine lettuce | 9 | 5 (55.6) | Â | |||
Sudan | Lugol’s iodine stain | Tomatoes | 36 | 1 (2.8) |  | [29] |
Cucumber | 12 | 0 | Â | |||
Armenian cucumber | 16 | 0 | Â | |||
Green pepper | 25 | 1 (4.0) | Â | |||
Cayenne pepper | 7 | 0 | Â | |||
Radish | 24 | 1 (4.2) | Â | |||
Beet | 19 | 0 | Â | |||
Watercress | 23 | 2 (8.7) | Â | |||
Lettuce | 11 | 1 (9.1) | Â | |||
Green onion | 36 | 1 (2.8) | Â | |||
Carrot | 50 | 1 (2.0) | Â | |||
Total | Â | Â | 5739 | 276 (4.8) | Â | Â |