Fig. 2

Identification of Leishmania species from biopsy samples. PCR amplification of Leishmania genomic DNA from fresh biopsy samples using the newly developed universal primers UNIL-IR-P and UNIL-IR-M subjected to electrophoresis on a 1.5% agarose gel. Lane 1: amplicon of L. major MRHO/IR/75/ER reference strain; Lane 2: amplicon of a positive L. major sample; Lane 3: amplicon of L. tropica MHOM/SU/74/K27 reference strain; Lane 4: amplicon of a positive L. tropica sample; Lane 5: amplicon of L. infantum MCAN/IR/96/LON49 reference strain; Lanes 6–8: negative biopsy samples that mimic CL, including fungal skin infection, tuberculosis and skin neoplasm, respectively. Lane 9: non-template negative control. Lane L: 100 bp DNA ladder