Table 1 Characteristics of PCR primer pairs
Strategy | Name | Species | Primer sequence (5′–3′) | GenBank ID | Nucleotide no. | Size (bp) | |
|---|---|---|---|---|---|---|---|
Forward | Reverse | ||||||
Multiplex PCR | MULt-IR-P and MULt-IR-M | L. tropica | ACGCACCGCCTATACACAAA | ACTACTGCGTTCTTCACCGA | MH627386.1 | 138–292 | 155 |
MULm-IR-P and MULm-IR-M | L. major | TCCGATGCTTACACCCCAAA | ATGCACGGGGATGACACAAT | KU680845.1 | 16–421 | 406 | |
MULi-IR-P and MULi-IR-M | L. infantum | ACATATACAACTCGGGGAGACC | AGGAAGCCAAGTCATCCATCG | KU975159.1 | 34–274 | 241 | |
Universal PCR | UNIL-IR-P and UNIL-IR-M | L. major, L. tropica, L. infantum | CATGCCATATTCTCAGTGTCG | GGTCTGTAAACAAAGGTTGTCG | – | – | L. m: 740; L. inf: 690; L. t: 640 |