Fig. 2

The BI phylogenetic tree of Bulinus species with bars, on the right, denoting different species delimitation results, based on the dataset of concatenated cox1 and ITS2 sequences. Within the phylogeny, nodes supported and shared between BI and ML methods are marked with stars where support equates to 90–100% (ML) and 0.95–1 (BI). Names in bold are for specimens collected in the present study and the rest have been retrieved from GenBank: B. truncatus (a); B. tropicus (b); Bulinus sp. 1 (c); Bulinus sp. 2 (d). Locality details are provided in Table 1. Abbreviations: CLI, Clade I; CLII, Clade II; SCI, Subclade I; SCII, Subclade II. The three-letter abbreviation for countries is also given. Notes: the blue colour represents different species, while green stands for the same species according to species delimitation methods. The three-letter abbreviations represent countries: NIG, Nigeria; SAF, South Africa; UGA, Uganda; MLW, Malawi; TZA, Tanzania; CAM, Cameroon; SEN, Senegal; ZNZ, Zanzibar; KEN, Kenya; ANG, Angola. The information for sequences retrieved from the GenBank is presented in Additional file 1: Table S1