Fig. 2

Analysis of the specific humoral responses before and six weeks after challenge. Before injection of the wild parasite (L. major (MRHO/IR/75/ER)), sera from all groups were evaluated for anti-L. tarentolae-LACK/KMP11/EGFP F/T antigen using ELISA. At 6 weeks after the challenge, L. major F/T antigen was tested for all groups. a, b Assessment of IgG1 levels in sera collected from groups of mice. c, d IgG2a. All antibody ODs represent as Mean ± SD in each bar. e, f The ratio of IgG2a/IgG1. Statistical analysis of the data was performed with Mann-Whitney U-test (*P < 0.05, **P < 0.001, ***P < 0.0001; ns, non-significant)