Fig. 6

Measurement of footpad induration, ulceration and parasite load with Real-time PCR. Assessment of footpad induration (a) and ulceration (b) in vaccinated and control groups. The mean ± SD footpad induration and ulceration measurements calculated by vernier caliper in mm. BALB/c mice were immunized subcutaneously with L. tarentolae-LACK/KMP11/EGFP + CpG (G1, rLive vaccine); L. tarentolae-LACK/KMP11/EGFP + non-CpG (G2, rLive vaccine,); L. tarentolae-EGFP + CpG (G3, control); L. tarentolae-EGFP + non-CpG (G4, control); mice were injected with PBS (G5, control). One-way ANOVA with Tukeyʼs multiple comparison post-hoc tests were used for statistical analysis (the asterisk indicates the significant difference between values at the indicated time points). c–f Quantification of parasite burden in the popliteal lymph nodes (c, d) and in the footpad (e, f) in all groups at 8 and 11 weeks after the challenge was estimated by real-time PCR. All reactions were performed in triplicate. Statistical analysis of data was performed with Mann-Whitney U-test. (*P < 0.05, **P < 0.001, ***P < 0.0001; ns, non-significant)