Frequency of kdr mutations in the voltage-sensitive sodium channel (VSSC) gene in Aedes aegypti from Yogyakarta and implications for Wolbachia-infected mosquito trials

Table 2 Primers to amplify V_SSC mutations of Aedes aegypti from the city of Yogyakarta, Indonesia

Mutation	Region amplified	Sequence (5’-3’)	Product size (bp)
V1016G	IIS6 first codon of exon 21	GACAAATTGTTTCCCACCCGCACAG	52
V1016G	IIS6 first codon of exon 21	AAGCAAGGCTAAGAAAAGGTTAAG	52
F1534C	IIIS6 24th codon of exon 31	TACCTCTACTTTGTGTTCTTCATCATC	52
F1534C	IIIS6 24th codon of exon 31	GATTCAGCGTGAAGAACGACCCG	52
S989P	IIS5 S6 first codon of P region	CGGGTATTATGCGGCGAGTGGATC	52
S989P	IIS5 S6 first codon of P region	CCCACAAGCATACAAT CCCACATGG	52

ISSN: 1756-3305