Table 1 Primer sequences used in qPCR
Target mRNA | Primers | |
|---|---|---|
Forward | Reverse | |
TLR1 | GGTGTTGGCTGTGACTGTGA | TGGAGTTCTTCTAAGGGTATG |
TLR2 | GATGCCTACTGGGTGGAGA | AGACGGAAATGGGAGAAGT |
TLR3 | CCAAGCCTTCAACGACTG | TTGCGTGTTTCCAGAGCC |
TLR4 | CCGCTTCCTGGTCTTATCAT | TCTGCTGCAACTCATTTCAT |
TLR5 | CAACCTTACAGCGAACC | AAACATCCCAACAGAGC |
TLR6 | CAGTTAATACTTTAGGGTGCT | CGTTTCTATGTGGTTGAGGG |
TLR7 | CCTTTCCCAGAGCATACAGC | GGACAGAACTCCCACAGAGC |
TLR8 | CAGAGCATCAACCAAAGCAA | GCTGCCGTAGCCTCAAATAC |
TLR9 | GTGCAGCCGGAGATGTTT | CGTGAATGAGTGCTCGTGGTAG |
TLR10 | GCCCACCACAATCTCTTCCA | GCCCACATTTACGCCTATCCTT |
GAPDH | AGAAGGCTGGGGCTCATTTG | AGGGGCCATCCACAGTCTTC |
U6 | GCTTCGGCAGCACATATACAAAAT | CGCTTCACGAATTTGCGTGTCAT |
β-actin | AGCGAGCATCCCCCAAAGTT | GGGCACGAAGGCTCATCATT |