Fig. 3
Antioxidant activity of SjPrx-4. Lane 1: pBluescript plasmid DNA; Lane 2: pBluescript plasmid DNA+ FeCl3; Lane 3: pBluescript plasmid DNA+DTT; Lane 4: pBluescript plasmid DNA + FeCl3 + DTT; Lanes 5–8: pBluescript plasmid DNA + FeCl3 + DTT and 10 ng, 100 ng, 1 µg, and 5 µg/ml of rSjPrx-4, respectively; Lanes 9, 10: pBluescript plasmid DNA + FeCl3 + DTT and 1 µg, and 5 µg/ml of denatured rSjPrx-4, respectively. The nicked form (NF) and supercoiled form (SF) are indicated on the right