Fig. 4

ATM/CHK2 activation upon T. gondii infection. a HeLa cells were infected with T. gondii RHΔku80 at a MOI of 10:1. Expression of ATM, ATM-S1981, CHK2 and CHK2-T68 were detected by western blot at 0 h (uninfected control), 10 h, 20 h or 30 h post-infection. All antibodies were used at a 1:1000 dilution. b Quantitation of ATM-S1981 shown in a with ATM-S1981 levels of uninfected cells set as one arbitrary unit. c Quantitative data of CHK2-T68 shown in a with CHK2-T68 levels of uninfected cells set as one arbitrary unit. Data are presented as the mean ± SD (n = 3). All experiments were performed in triplicate. ***P ≤ 0.001; ns: not significant