Fig. 1

Alignment and phylogenetic tree of multiple NbPTP6 homologous sequences in various microsporidia, fungi and bacteria. a Phylogenetic tree of multiple NbPTP6 homologous sequences. Neighbour-joining tree was constructed using MEGA 5 software. The amino acid sequences were obtained from Uniprot: E. cuniculi (AEWD_081700); E. hellem (EHEL_081670); N. ceranae (NCER_100577); V. culicis (VCUG_01246); Spr. lophii (SLOPH_1767); N. bombycis (NBO_1135g0001); E. romaleae (EROM_081710); E. intestinalis (Eint_081680); Smi. culicis (AYI70_g11826); P. brasiliensis (PADG_06126); and Ser. vermifera (M408DRAFT_280892). b Alignment of NbPTP6 homologous sequences. The red shades highlight identical amino acids; the red underline indicates the predicted signal peptide of NbPTP6; the blue and green triangles represent O-glycosylation sites and N-glycosylation sites of NbPTP6, respectively