Fig. 3

Immunohistochemical staining with primary anti-TLR2 (a–h) and anti-TLR4 antibodies (i–p) in the kidneys of immunocompetent and immunosuppressed mice from control groups (0 dpi) and at 8, 16, and 24 dpi. Magnification 40×. In the immunocompetent and immunosuppressed uninfected mice, TLR2 expression was found in the proximal tubules (white arrows) (a, e), while after Acanthamoeba spp. infection brown pigmentation was observed in the distal tubules and collecting ducts (black and red arrows, respectively) (a–h). The immunoexpression of TLR4 was observed in the proximal and distal tubules (white and black arrows, respectively), collecting ducts (red arrows) and in the renal corpuscles (yellow arrows) (i–p). The immunosuppressed mice after 16 days post-Acanthamoeba spp. infection had immunopositive nuclei in the epithelial cells of the distal tubule (black asterisk) and the highest intensity of TLR4 immunoreaction the intensity of TLR4 immunoreaction (o). dpi days post-Acanthamoeba spp. infection, IC immunocompetent mice, IS immunosuppressed mice