Field-deployable molecular diagnostic platform for arbovirus detection in Aedes aegypti

Parasites & Vectors

Table 3 Detection of viral RNA in pooled Aedes aegypti samples by bCUBE qRT-PCR

	Pool size
	5			10			15			20
	Titer of individual mosquito^a	Titer of pool^a	No. of positives	Titer of individual mosquito^a	Titer of pool^a	No. of positives	Titer of individual mosquito^a	Titer of pool^a	No. of positives	Titer of individual mosquito^a	Titer of pool^a	No. of positives
ZIKV	47	15	3/3	52	9	3/3	6666	45	3/3	58	8	3/3
	4181	88		17,095	68		10,721	239		27,257	641
	19,629	1808		383,403	1989		193,745	4480		390,085	10,629
DENV2	760	34	3/3	49	2	3/3	89	1	3/3	47	UDL	2/3
	1471	17		618	48		1846	3		12,844	54
	152,817	1682		21,077	14		27,848	157		59,144	279

^aRNA copy number determined by reverse transcriptase-polymerase chain reaction standard curve
Notes: Pooled samples were prepared by combining individual ZIKV- and DENV2-infected mosquitoes with pools of uninfected mosquitoes. Pools of 4, 9, 14, or 19 uninfected mosquitoes plus one infected mosquito were evaluated by bCUBE qRT-PCR. Table lists number of positive pooled samples detected by bCUBE qPCR and the titer of individually infected mosquitoes and corresponding pooled samples
Abbreviation: UDL, under detection limit

ISSN: 1756-3305