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Table 3 Detection of viral RNA in pooled Aedes aegypti samples by bCUBE qRT-PCR

From: Field-deployable molecular diagnostic platform for arbovirus detection in Aedes aegypti

  Pool size
5 10 15 20
Titer of individual mosquitoa Titer of poola No. of positives Titer of individual mosquitoa Titer of poola No. of positives Titer of individual mosquitoa Titer of poola No. of positives Titer of individual mosquitoa Titer of poola No. of positives
ZIKV 47 15 3/3 52 9 3/3 6666 45 3/3 58 8 3/3
4181 88 17,095 68 10,721 239 27,257 641
19,629 1808 383,403 1989 193,745 4480 390,085 10,629
DENV2 760 34 3/3 49 2 3/3 89 1 3/3 47 UDL 2/3
1471 17 618 48 1846 3 12,844 54
152,817 1682 21,077 14 27,848 157 59,144 279
  1. aRNA copy number determined by reverse transcriptase-polymerase chain reaction standard curve
  2. Notes: Pooled samples were prepared by combining individual ZIKV- and DENV2-infected mosquitoes with pools of uninfected mosquitoes. Pools of 4, 9, 14, or 19 uninfected mosquitoes plus one infected mosquito were evaluated by bCUBE qRT-PCR. Table lists number of positive pooled samples detected by bCUBE qPCR and the titer of individually infected mosquitoes and corresponding pooled samples
  3. Abbreviation: UDL, under detection limit