Fig. 2

Expression and immune response of rHlSu. a Expression and purification of recombinant Haemaphysalis longicornis subolesin (rHlSu) using an E. coli expression system. Each protein (Lanes 1–4, 6 μg; Lane 5, 2.25 μg) was applied and separated by SDS-PAGE. Lane M: protein marker; Lane 1, empty vector without IPTG induction; Lane 2, empty vector with IPTG induction; Lane 3, vector including subolesin without IPTG induction; Lane 4, vector including subolesin with IPTG induction; Lane 5, purified rHlSu. b The immune response of rHlSu was verified using Western blotting. Purified rHlSu (0.03 μg) was used for Western blotting. Serum dilutions were 1:600 for lanes anti-H. longicornis serum and Naïve serum, and 1:2000 for lane anti-rHlSu serum. Secondary antibody dilution was 1:5000 for all lanes. Anti-Hl, reaction with anti-H. longicornis serum; Anti-rHlSu, reaction with anti-rHlSu serum; Naïve, reaction with naïve serum