Fig. 2
From: Vectors, molecular epidemiology and phylogeny of TBEV in Kazakhstan and central Asia
Phylogenetic tree illustrating the evolutionary relationships based on the nucleotide (nt) sequences for the envelope gene (E). The trees were constructed with the MEGA7 software package using the Neighbor-Joining method [79, 80]. The percentage of replicate trees are shown next to the branches, bootstrap test was made with 10,000 replicates [81]. Omsk hemorrhagic fever virus (GenBank: AF482341) was used as the outgroup. In the nt tree, the lineages of the Siberian subtype (Vasilchenko, Zausaev, Baltic and Obskaya) are highlighted. The newly generated sequences from Kazakhstan are marked with squares (Tekeli), circles (Talgar) and triangles (Yenbekshikazakh). The previously published TBEV sequences from Kazakhstan are marked with diamonds