Table 1 PCR primers used to amplify and sequence the mitochondrial genome of the rat lice, Hoplopleura sp.
Primer | Sequence (5′–3′) | Minichromosome |
|---|---|---|
1F | AGCACTTGTTCTGATTCTTCGGTC | I-cox1 |
1R | TCGTGATACCCCCTGCCAAAACTG | I-cox1 |
2F | CTTTCAAGAGACACAAGGGGTTCA | rrnS |
2R | TATTTTCCCAGTCCTACAGAGAGC | rrnS |
3F | TGTCCTTGTCCCGAAAGAGAGTGAT | M-L1-rrnL-V |
3R | CTATTCCACCCTCCCTGATACAAAA | M-L1-rrnL-V |
4F | TGAGTAAGGGGGATACATCACGCTA | Q-nad1-G-nad3 |
4R | CAGCGAACTCTGCGTATTCCTCCAT | Q-nad1-G-nad3 |
5F | TAAGGTTATCGGGCATCAGTGGTA | D-Y-cox2-T |
5R | AGAGGGGATGGCGAGGACAAAAAG | D-Y-cox2-T |
6F | CGCCAACTATCAGAACTTTCCAAC | atp8-atp6-N |
6R | TCGTGGATAACAGTCACAAAGATG | atp8-atp6-N |
7F | GCATTTACAGTGCTCAGTCTTCGC | nad2 |
7R | ACAAAGACAAAGGGGGAAACGGGA | nad2 |
8F | TTAGCGGTAAGCGGGACTGAGGTA | C-nad6-W-L2 |
8R | AACTCTATTTCCCCCGTTTCCCAA | C-nad6-W-L2 |
9F | GTTCCTCTCGGTTTTCCATCCCTCA | R-nad4L-P-cox3-A |
9R | TCTATCGCTACCAGAGAGATTGTTA | R-nad4L-P-cox3-A |
10F | GGGAAAACTCCGACAAGGTCACATT | E-cytb-S1-S2 |
10R | CCTAAGGGATTTGAACTTCCTGTCG | E-cytb-S1-S2 |
11F | GGTATTGCTAAAGTTTGGAGGTATC | K -nad4 |
11R | CAGCCAAGAGTATTCTCCCCAACAT | K -nad4 |
12F | GGGGATTACCTCCTTCCTTCTCATT | H-nad5-F |
12R | AAGCAATGAAGAGCAACAAGGACAC | H-nad5-F |