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Fig. 8 | Parasites & Vectors

Fig. 8

From: Transcription factor FTZ-F1 regulates mosquito cuticular protein CPLCG5 conferring resistance to pyrethroids in Culex pipiens pallens

Fig. 8

Silencing of FTZ-F1 reduced the expression of CPLCG5 protein. a Schematic diagram of experimental time course for siRNA microinjection and IF. b Illustration of the position of sectioning on the Cx. pipiens pallens tarsomere 1 (t1-t5 = five tarsal segments [52]). The red line indicates in which leg part the sections were taken. c Representative images of CPLCG5 localization in the tarsi of the siNC and siFTZ-F1 groups. Each IF panel comprises three images, with red fluorescence of CPLCG5 on the left, nuclei stained with DAPI in the middle, and merged photos on the right. d Quantification of CPLCG5 protein levels in the tarsi of the siNC and siFTZ-F1 groups using Image-Pro Plus (IPP) software. *P < 0.05 indicates a significant difference. Results are shown as the mean ± SD. n = the number of measures carried out on each batch of 25 mosquitoes (siNC & siFTZ-F1)

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