Fig. 3
Establishing the Whip-LAMP assay. a LAMP results obtained using the set-up Whip-LAMP visualized with the addition of SYBR Green I (up) or agarose gel (down). Lane M: molecular weight marker (100 bp Plus Blue DNA Ladder); Lane Tm: DNA of T. muris (20 ng); Lane N: negative control (ultrapure water instead DNA template). b Detection limit of the Whip-LAMP assay using serial dilutions of T. muris genomic DNA. Lane M: molecular weight marker (100 bp Plus Blue DNA Ladder); Lane Tm: DNA of T. muris (20 ng); Lanes 10−1–10−10: 10-fold serial dilutions of T. muris DNA; Lane N: negative control (ultrapure water instead DNA template). c Specificity of the Whip-LAMP assay. Lane M: molecular weight marker (100 bp Plus Blue DNA Ladder); Lane Tm: DNA of T. muris; Lanes Sm, Sh, Fh, Amp, Eg, Tsag, Tsol, Dd, Hd, Ans, Bp, Loa, Mp, Sv, As, Gd, Cp and Eh: DNA samples of Schistosoma mansoni, S. haematobium, Fasciola hepatica, Amphimerus sp., Echinococcus granulosus, Taenia saginata, T. solium, Dicrocoelium dendriticum, Hymenolepis diminuta, Anisakis simplex, Brugia pahangi, Loa loa, Mansonella perstans, Strongyloides venezuelensis, Ascaris suum, Giardia duodenalis, Cryptosporidium parvum and Entamoeba histolytica, respectively; Lane N: negative controls (ultrapure water instead of DNA template)