Fig. 2

Genomic DNA PCR to confirm gene disruption. a Genomic DNA PCR to confirm the successful modification of the Pfhrp2 gene by the sgRNA. b Genomic DNA PCR to verify hrp2 deletion in monoclonal parasites. 3D7 Wild-type strain of Plasmodium falciparum, L, L2 transgenic parasites that were disrupted in exon 1 plus exon 2 and only exon 2 of Pfhrp2, respectively. PCR product sizes of the whole fragment (w) and partial fragment (s) were 2903 and 725 bp, respectively