Fig. 1
From: Detection of Leishmania infantum DNA in Pintomyia evansi and Lutzomyia longipalpis in Honduras
Polymerase chain reaction (PCR) to determine Leishmania spp. infection using Leish1-Leish2 primers to target conserved DNA regions of the kinetoplast DNA from Leishmania spp. [120 base pairs (bp)]. Lane M Molecular weight marker (100-bp DNA ladder). Lanes 1–16 Female sand fly DNA [lanes 1-10 Lutzomyia (Lutzomyia) longipalpis, positive female; lanes 11, 13–16 Pintomyia (Pifanomyia) evansi, positive female]. Lane 17 PCR positive control [DNA extracted from a mixture of the male insect pool containing Leishmania (Leishmania) infantum DNA]. Lane 18 Amplification reaction without added DNA (PCR negative control)