Fig. 4a, b

Longevity of progeny of females fed different sources of blood. Survival of Ae. aegypti infected with wMelBr (green curves) was compared with that of naturally uninfected and uninfected laboratory strains (blue curves). For each strain, three replicate groups of 25 male (a) or female (b) mosquitoes were maintained at 25 °C, 70–80% relative humidity, under a 12:12-h light:dark photoperiod. Three independent experiments were carried out in each case. There was no significant difference in male longevity according to one-way ANOVA followed by Tukey’s multiple comparison test (F = 2.4, P = 0.06). Regarding the females, there was no significant difference between the controls [Wolbachia-free mosquitoes of the Rockefeller lineage (Rock) × wMelBr] or among the Wolbachia strains. However, there was a significant difference between the progenies of infected mosquitoes and the controls according to one-way ANOVA followed by Tukey’s multiple comparison test (F = 10.57, P < 0.05)