Fig. 2

Structure and sequence analysis of Acan-RPS-S30. a The exon–intron organization of Acan-rps-30. The Acan-rps-30 gene, from A. cantonensis, spans 2967 bp and consists of 4 exons and 3 introns. The narrow bar represents untranscribed sequences or introns; the wide bars represent exons; brown blocks are coding regions; gray blocks are the non-coding 5′- and 3′-UTR. ORF Open reading frame. b Alignment of amino acid sequences of Acan-RPS-S30 with those from Homo sapiens (Hs-RPS-30) and Caenorhabditis elegans (Ce-RPS-30). The accession numbers of sequences available from current databases are: NP_505007.1 (Ce-RPS-S30) and NP_001988.1 (Hs-RPS-30). Identical and similar residues are shown in black and gray blocks, respectively. The potential cleavage sites (Gly-Gly) of the fusion protein (ubiquitin-like [UBiL]-ribosome protein S30 [S30]) are indicated with green arrow (upstream and downstream sequences are UBiL and S30 regions, respectively). The nuclear location signals in the S30 regions are indicated by a green line underneath the sequences. The secondary structural elements of Acan-RPS-S30 are shown above the alignment. c Predicted tertiary structure of UBiL region, showing 3 β-sheets and 2 α-helixes. d Predicted tertiary structure of S30 region, showing 2 α-helixes