Fig. 4

Transcriptional profile of Acan-rps-30 in different developmental stages (third- and fifth-stage larvae [L3, L5, respectively], adult) and in different genders (females [F] and males [M]] of A. cantonensis, determined by real-time PCR analysis. Data shown are the mean ± standard error of the mean from three technical replicates with two biological replicates. Relative transcription of the Acan-rps-30 gene in each sample was calculated by normalization of the raw data, followed by the determination of abundance relative to the 18S ribosomal RNA gene (GenBank: AY295804), which served as an internal loading control. Statistical analysis was conducted using a one-way analysis of variance. Asterisks indicate statistical difference at *P < 0.05 and **P < 0.01