Fig. 6

Cross-species expression of Acan-RPS-30 in C. elegans N2 strain and the rps-30 deletion mutant worms. a–d Expression of pCe-rps-30::Acan-rps-30::rfp in C. elegans N2 strain. RFP was expressed widely, but was RFP mainly focused on the nucleus; “button-like” apoptotic corpses were seen in the anterior pharynx. Arrowheads indicate apoptotic corpses. e–g The heterozygous rps-30^+/–worm. The GFP fluorescence-positive worms (pharynx) carried the translocational balancer (nT1). h The homozygous rps-30^−/− worm. Worms without GFP (nT1) were mutation homozygous (rps-30^−/−). i–l Expression of pCe-rps-30::Acan-rps-30::rfp in the heterozygous rps-30^+/− worm. RFP was expressed widely, and GFP fluorescence was positive in pharynx. m–p Expression of pCe-rps-30::Acan-rps-30::rfp in the homozygous rps-30^−/− worm. RFP was expressed widely, and GFP fluorescence was negative in pharynx. q–s Expression of pCe-rps-30::gfp in the homozygous rps-30^−/− worm