Differential vector competence of Ornithodoros soft ticks for African swine fever virus: What if it involves more than just crossing organic barriers in ticks?

Table 1 Description of biological parameters used for correspondence analysis

Biological parameter	Name of biological parameter used in analysis	Method used to obtain results	Categories
Intestine	intestine_neg	qPCR	Negative ASFV PCR
	intestine_1		Cq between 45 and 35
	intestine_2		Cq between 35 and 25
	intestine_3		Cq <r 25
Salivary glands	sg_neg	qPCR	Negative ASFV PCR
	sg_1		Cq between 45 and 35
	sg_2		Cq between 35 and 25
	sg_3		Cq < 25
Reproductive organs	ro_neg	qPCR	Negative ASFV PCR
	ro_1		Cq between 45 and 35
	ro_2		Cqt between 35 and 25
	ro_3		Cq < 25
Viral titer	titer_neg	Viral titration	Viral titer between 10⁰ and 10²
	titer_1		Viral titer between 10² and 10⁴
	titer_2		Viral titer between 10⁴ and 10⁶
	titer_3		Viral titer between 10⁰ and 10²
	titer_4		Viral titer over 10⁶
Replication rates	up_qpcr	qPCR	Significant increase in viral replication
	down_qpcr		Significant decrease in viral replication
	no_qpcr		No significant variation in viral replication

ASFV, African swine fever virus; Cq, quantitative cycle (PCR); qPCR quantitative PCR

ISSN: 1756-3305