Detection of bluetongue virus in Culicoides spp. in southern Yunnan Province, China

Parasites & Vectors

Table 3 Detection of bluetongue virus seg10 RNA by reverse transcription-quantitative PCR from individual specimens of Culicoides species from Yunnan Province, China

Species	Source^a	Number of midges tested	RT-qPCR test results (values are number of midges)^b				Percentage of infected specimens (Cq ≤ 25)
Species	Source^a	Total	Cq ≤ 25	25 < Cq ≤ 30	Cq > 30	Non- reactive	Percentage of infected specimens (Cq ≤ 25)
C. jacobsoni	JH, YY	85	2	0	0	83	2.35
C. imicola	YY	40	2	0	0	38	5.0
C. tainanus	JC	105	1	0	3	101	0.95
Trithecoides	JH, JC, YY	250	0	0	1	249	0
C. oxystoma	JH, JC, YY	105	0	0	0	105	0
C. arakawai	JH, JC, YY	60	0	0	0	60	0
C. orientalis	JH, JC, YY	38	0	0	2	36	0
C. guttifer	JH, JC	31	0	0	0	31	0
C. spiculae	JC	24	0	0	2	22	0
C. insignipennis	JH	8	0	0	0	8	0
C. liui	JH	2	0	0	0	2	0
Total		748	5	0	8	748	0.67

RT-qPCR, Reverse transcription-quantitative PCR
^aJC, Jiangcheng; JH, Jinghong; YY, Yuanyang
^bFrom a pool of 8 specimens with a collective quantification cycle (Cq) value of < 35, indicating possible positivity, individual specimens were tested, using the same RT-qPCR. Lysate from individual specimens which produced a Cq value of < 25 was considered to indicate positivity for infection. Numbers indicate the number of specimens testing at the indicated Cq

ISSN: 1756-3305