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Fig. 3 | Parasites & Vectors

Fig. 3

From: A novel assay to isolate and quantify third-stage Dirofilaria immitis and Brugia malayi larvae emerging from individual Aedes aegypti

Fig. 3

A fraction of ingested microfilariae develops into eL3 in Aedes aegyptiS (a D. immitis- and Brugia malayi-susceptible strain). a Dots indicate the number of microfilariae present in the midgut of individual Ae. aegyptiR (a D. immitis- and B. malayi-resistant strain) immediately after feeding on infected blood (Uptake) and the number of eL3 assayed 14 days post infection (dpi). The black line indicates the median. b Dots indicate the number of microfilariae present in the midgut of individual Ae. aegyptiS immediately after feeding on infected blood (Uptake), the number of eL3 assayed 14 dpi (eL3), the number of L3 emerging from the dissected heads (Head) or carcass (Carcass) and the number present in the Malpighian tubules (MT). The Total is sum of all parasites of any stage found in any tissue or assay on 14 dpi. The red line indicates the median. c Dots are the number of eL3 from individual Ae. aegyptiS and all third-stage larvae assayed (Total; sum of eL3, Head, and Carcass) (data taken from panel b). The red line is the median number. The asterisks indicate a Mann–Whitney P value < 0.001. These data are the sum of three independent biological replicates. The number of mosquitoes assayed is shown below each column

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