Table 1 Summary of the experiments in relation to research questions
TEST treatment | CONTROL treatment | Human landing catch | Test and control independent or in competitiona | |
|---|---|---|---|---|
Experiment 1 | ||||
What is the human biting rate of Anopheles arabiensis released in the semi-field systems between 19.00 and 23.00 h in the absence of any treatment? | ||||
Are the two semi-field systems comparable in the results they generate? | ||||
Are there any differences in catching efficiency/attractiveness of HLC volunteers? | ||||
1.1 | No treatment | No treatment | Yes | Independent |
Experiment 2 | ||||
Can Citriodiol® and/or transfluthrin-treated strips located at eave gaps reduce An. arabiensis biting rates compared to untreated controls? | ||||
2.1 | 1 g/m2 microencapsulated Citriodiol® | Untreated cotton fabric | Yes | Independent |
2.2 | 11 g/m2 microencapsulated Citriodiol® | Untreated cotton fabric | Yes | Independent |
2.3 | 1.25 g/m2 transfluthrin fabric | Untreated hessian fabric | Yes | Independent |
2.4 | 2.5 g/m2 transfluthrin fabric | Untreated hessian fabric | Yes | Independent |
Experiment 3 | ||||
How effective is the MB5 baited Suna trap in attracting insectary-reared An. arabiensis in a large semi-field system in the absence and presence of a human being? | ||||
How does the MB5 cartridge perform in comparison to nylon strips impregnated by investigator? | ||||
Does 2-butanone combined with MB5 perform equally well in attracting insectary-reared An. arabiensis in a large semi-field system as CO2 produced from molasses fermentation? | ||||
How does the trapping efficacy compare between Suna traps baited with CO2only and Suna traps baited with the synthetic MB5 lure in addition to CO2? | ||||
3.1 | MB5-cartridge baited Suna trap supplemented with CO2 | MB5-nylon strip baited Suna trap supplemented with CO2 | No | Competing |
3.2 | MB5-cartridge baited Suna trap with 2-butanone | MB5-cartridge baited Suna trap with CO2 | No | Competing |
3.3 | MB5-cartridge baited Suna trap with 2-butanone | Unbaited Suna trap (no MB5, no CO2, only suction fan) | No | Competing |
3.4 | MB5-cartridge baited Suna trap with CO2 | Unbaited Suna trap (no MB5) supplemented with CO2 only | No | Competing |
3.5 | MB5-cartridge baited Suna trap supplemented with CO2 | Unbaited Suna trap (no MB5, no CO2, only suction fan) | Yes | Independent |
3.6 | MB5-cartridge baited Suna trap with 2-butanone | Unbaited Suna trap (no MB5, no CO2, only suction fan) | Yes | Independent |
Experiment 4 | ||||
Can Citriodiol® and/or transfluthrin-treated strips located at eave gaps reduce An. arabiensis biting rates compared to untreated controls? | ||||
4.1 | Transfluthrin 2.5 g/m2 eave wrap + MB5-cartridge and with CO2 | Untreated eave wrap + unbaited Suna trap | Yes | Independent |